Ukulingisa izimo zomzimba kusiza abacwaningi bathole izibophezelo zensimbi
Abacwaningi baye benza indlela yokubona ama-molecule amancane abopha ama-ion ensimbi.Ama-ion ensimbi abalulekile kubhayoloji.Kodwa ukuthola ukuthi yimaphi ama- molecule—ikakhulukazi imaphi ama- molecule amancane—lawo ma-ion ensimbi asebenzisana nawo kungaba inselele.
Ukuhlukanisa ama-metabolites ukuze ahlaziywe, izindlela ezivamile ze-metabolomics zisebenzisa izinyibilikisi eziphilayo kanye nama-pH aphansi, angabangela ukuba izinsimbi zensimbi zihlukane.U-Pieter C. Dorrestein wase-University of California San Diego kanye nozakwabo bafuna ukugcina izakhiwo zihlangene ukuze zihlaziywe ngokulingisa izimo zomdabu ezitholakala kumaseli.Kepha uma besebenzisa izimo zemvelo ngesikhathi sokuhlukaniswa kwama-molecule, bekuzodingeka balungise kabusha izimo zokuhlukaniswa kwesimo ngasinye sokuphila abafuna ukusihlola.
Esikhundleni salokho, abacwaningi bathuthukise indlela yezigaba ezimbili eyethula izimo ze-physiological phakathi kokuhlukaniswa kwe-chromatographic evamile kanye nokuhlaziywa kwe-spectrometric enkulu (Nat. Chem. 2021, DOI: 10.1038 / s41557-021-00803-1).Okokuqala, bahlukanise ukukhishwa kwezinto eziphilayo kusetshenziswa i-chromatography yoketshezi esebenza kahle kakhulu.Babe sebelungisa i-pH yokugeleza okuphuma kukholamu ye-chromatographic ukuze balingise izimo zomzimba, bengeza ama-ion ensimbi, futhi bahlaziya ingxube nge-mass spectrometry.Basebenzisa ukuhlaziya kabili ukuze bathole i-spectra enkulu yama-molecule amancane anezinsimbi nangenawo.Ukuze bakhombe ukuthi yimaphi ama-molecule abopha izinsimbi, basebenzise indlela yokubala esebenzisa umumo ophezulu ukuze baqonde ukuxhumana phakathi kohlaka lwezinguqulo ezibophekile nezingaboshiwe.
Enye indlela yokuqhubekisela phambili ukulingisa izimo zomzimba, u-Dorrestein uthi, kungaba ukwengeza ukugxila okuphezulu kwama-ion njenge-sodium noma i-potassium nokugxila okuphansi kwensimbi enentshisekelo.“Kuba ukuhlolwa komncintiswano.Izokutshela ukuthi, KULUNGILE, le molekyuli ngaphansi kwalezo zimo inokuthambekela okwengeziwe kokubopha i-sodium ne-potassium noma le nsimbi eyodwa eyingqayizivele oyengezile, ”kusho u-Dorrestein."Singakwazi ukufaka izinsimbi eziningi ezahlukene ngesikhathi esisodwa, futhi singakuqonda ngempela okuthandwayo nokukhetha kulowo mongo."
Ekukhishweni kwesiko ku-Escherichia coli, abacwaningi bahlonze izinhlanganisela ezaziwayo zokubopha insimbi njenge-yersiniabactin ne-aerobactin.Endabeni ye-yersiniabactin, bathole ukuthi ingaphinda ibophe i-zinc.
Abacwaningi bahlonze izinhlanganisela ezibopha insimbi kumasampuli njengeziyinkimbinkimbi njengezinto eziphilayo ezincibilikisiwe ezivela olwandle.“Lelo ingelinye lamasampula ayinkimbinkimbi kakhulu engake ngawabheka,” kusho uDorrestein.“Cishe iyinkimbinkimbi njengowoyela ongahluziwe, uma ungeyona inkimbinkimbi.”Indlela ihlonze i-domoic acid njenge-molecule ebopha ithusi futhi yaphakamisa ukuthi ibophe i-Cu2+ njenge-dimer.
"Indlela ye-omics yokuhlonza wonke ama-metabolites abopha insimbi kusampula iwusizo kakhulu ngenxa yokubaluleka kwe-biological metal chelation," u-Oliver Baars, ocwaninga ama-metabolites abopha insimbi akhiqizwa izitshalo namagciwane eNyuvesi yaseNorth Carolina State, ubhala encwadini. i-imeyili.
"UDorrestein kanye nozakwabo banikeza isivivinyo esihle, esidingeka kakhulu sokuhlola kangcono ukuthi ingaba yini indima engokomzimba yama-ion ensimbi esitokisini," kubhala u-Albert JR Heck, iphayona lokuhlaziya i-mass spectrometry yase-Utrecht University, kubhala i-imeyili."Isinyathelo esilandelayo esingaba khona kungaba ukukhipha ama-metabolite ngaphansi kwezimo zendabuko kuseli futhi uwahlukanise ngaphansi kwezimo zendabuko, ukuze ubone ukuthi yimaphi ama-metabolites aphethe ama-ion ensimbi yeselula."
Amakhemikhali Nezindaba Zobunjiniyela
I-ISSN 0009-2347
Copyright © 2021 American Chemical Society
Isikhathi sokuthumela: Dec-23-2021